Depth and extent of gold nanorod photothermal conversion in tissue-like phantoms that contain hemoglobin

Nisha Sharma; Astrid Chamson-Reig; Hazem Al-Aabed; Jeffrey J. L. Carson

doi:10.1117/12.860786

10 September 2010 Depth and extent of gold nanorod photothermal conversion in tissue-like phantoms that contain hemoglobin

Nisha Sharma, Astrid Chamson-Reig, Hazem Al-Aabed, Jeffrey J. L. Carson

Author Affiliations +

Proceedings Volume 7754, Metamaterials: Fundamentals and Applications III; 77541D (2010) https://doi.org/10.1117/12.860786
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

Gold nanorods (AuNRs) are of interest for many biomedical applications due to their tunable optical properties. AuNRs efficiently absorb light in the near-infrared (NIR) region, which induces effects such as hyperthermia and/or cell killing by localized microbubble formation through photothermal conversion. Our objective was to study the potential of AuNRs to elicit photothermal conversion effects due to pulsed laser exposure at depth within tissue-like phantoms. The approach was to measure photothermal conversion in inclusion-containing phantoms representative of breast cancer. Tissue-like phantoms were prepared with hemoglobin at 10 μM in a homogeneous mixture of 1% agarose and 1% Intralipid to mimic the optical properties of human breast tissue. Polyethylene glycol AuNR-loaded gel spheres (at an equivalent optical density of 0.67 at 800 nm) were prepared with hemoglobin at 20 μM in a homogeneous mixture of 1% agarose and 1% Intralipid. The spherical gel inclusions were cast into the phantom material at a depth of 0, 5, 10, or 15 mm. Phantoms were then exposed to nanosecond pulsed-NIR light (800 nm; 5 ns pulse duration; 17-100 mJ/cm²; 10- 1000 pulse count). Each phantom was then cut longitudinally and imaged with a NIR camera. The images were examined with image analysis software. Preliminary results indicated that the greatest extent of photothermal conversion occurred in spherical AuNR-loaded gels next to the phantom surface. Based on these results, we concluded that within ANSI limits of laser exposure photothermal therapy with AuNR-based agents will be limited to surface lesions and/or lesions accessible with needle-based light delivery.

Citation Download Citation

Nisha Sharma, Astrid Chamson-Reig, Hazem Al-Aabed, and Jeffrey J. L. Carson "Depth and extent of gold nanorod photothermal conversion in tissue-like phantoms that contain hemoglobin", Proc. SPIE 7754, Metamaterials: Fundamentals and Applications III, 77541D (10 September 2010); https://doi.org/10.1117/12.860786

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