An optical device employing multiwavelength photoplethysmography for non-invasive in-vivo monitoring of optically active nanoparticles

Gregory J. Michalak; Pratik Adhikari; Jon A. Schwartz; Glenn P. Goodrich; D. Patrick O'Neal

doi:10.1117/12.875089

10 February 2011 An optical device employing multiwavelength photoplethysmography for non-invasive in-vivo monitoring of optically active nanoparticles

Gregory J. Michalak, Pratik Adhikari, Jon A. Schwartz, Glenn P. Goodrich, D. Patrick O'Neal

Author Affiliations +

Proceedings Volume 7906, Optical Diagnostics and Sensing XI: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue III; 790604 (2011) https://doi.org/10.1117/12.875089
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Researchers employ increasingly complex sub-micron particles for oncological applications to deliver bioactive therapeutic or imaging compounds to known and unknown in vivo tumor targets. These particles are often manufactured using a vast array of compounds and techniques resulting in a complex architecture, which can be quantified ex vivo by conventional metrology and chemical assays. In practice however, experimental homogeneity using nanoparticles can be difficult to achieve. While several imaging techniques have been previously shown to follow the accumulation of nanoparticles into tumor targets, a more rapid sensor that provides a quantifiable estimate of dose delivery and short-term systemic response could increase the clinical efficacy and greatly reduce the variability of these treatments. We have developed an optical device, the pulse photometer, that when placed on an accessible location will estimate the vascular concentration of near-infrared extinguishing nanoparticles in murine subjects. Using a technique called multi-wavelength photoplethysmography, the same technique used in pulse oximetry, our pulse photometer requires no baseline for each estimate allowing it to be taken on and off of the subject several times during experiments employing long circulating nanoparticles. We present a formal study of our prototype instrument in which circulation half-life and nanoparticle concentration of gold nanorods is determined in murine subjects with the aid of light anesthesia. In this study, we show good agreement between vascular nanorod concentrations (given in optical density) as determined by our device and with UV-VIS spectrophotometry using low volume blood samples.

Citation Download Citation

Gregory J. Michalak, Pratik Adhikari, Jon A. Schwartz, Glenn P. Goodrich, and D. Patrick O'Neal "An optical device employing multiwavelength photoplethysmography for non-invasive in-vivo monitoring of optically active nanoparticles", Proc. SPIE 7906, Optical Diagnostics and Sensing XI: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue III, 790604 (10 February 2011); https://doi.org/10.1117/12.875089

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Nanorods

Nanoparticles

Blood

Photometry

Particles

Absorbance

Signal attenuation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years