Time-resolved fluorescence spectroscopic investigation of cationic polymer/DNA complex formation

Cosimo D'Andrea; Andrea Bassi; Paola Taroni; Daniele Pezzoli; Alessandro Volonterio; Gabriele Candiani

doi:10.1117/12.889643

10 June 2011 Time-resolved fluorescence spectroscopic investigation of cationic polymer/DNA complex formation

Cosimo D'Andrea, Andrea Bassi, Paola Taroni, Daniele Pezzoli, Alessandro Volonterio, Gabriele Candiani

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 80871J (2011) https://doi.org/10.1117/12.889643
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Since DNA is not internalized efficiently by cells, the success of gene therapy depends on the availability of carriers to efficiently deliver genetic material into target cells. Gene delivery vectors can be broadly categorized into viral and non-viral ones. Non-viral gene delivery systems are represented by cationic lipids and polymers rely on the basics of supramolecular chemistry termed "self-assembling": at physiological pH, they are cations and spontaneously form lipoplexes (for lipids) and polyplexes (for polymers) complexing nucleic acids. In this scenario, cationic polymers are commonly used as non-viral vehicles. Their effectiveness is strongly related to key parameters including DNA binding ability and stability in different environments. Time-resolved fluorescence spectroscopy of SYBR Green I (DNA dye) was carried out to characterize cationic polymer/DNA complex (polyplex) formation dispersed in aqueous solution. Both fluorescence amplitude and lifetime proved to be very sensitive to the polymer/DNA ratio (N/P ratio, +/-).

Citation Download Citation

Cosimo D'Andrea, Andrea Bassi, Paola Taroni, Daniele Pezzoli, Alessandro Volonterio, and Gabriele Candiani "Time-resolved fluorescence spectroscopic investigation of cationic polymer/DNA complex formation", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871J (10 June 2011); https://doi.org/10.1117/12.889643

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