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13 February 2012 Latest advances in commercially available STED microscopy
Wernher Fouquet, Arnold Giske
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Proceedings Volume 8228, Single Molecule Spectroscopy and Superresolution Imaging V; 82280W (2012) https://doi.org/10.1117/12.912751
Event: SPIE BiOS, 2012, San Francisco, California, United States
Abstract
STimulated Emission Depletion (STED) microscopy enables imaging of biological samples combining significantly improved optical resolution with all benefits of confocal microscopy. Especially, by combining multi-channel image acquisition with high spatial resolution opens up a new understanding of co-localization experiments on nanoscales. Such a microscope provides new insights in various fields of biology, such as cell and membrane biology, neurobiology and physiology. We present new developments and a variety of biological examples for STED microscopy, showing structural details on scales well below 70nm and give an overview of possible field of applications, mainly focused on live cell imaging.
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Wernher Fouquet and Arnold Giske "Latest advances in commercially available STED microscopy", Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280W (13 February 2012); https://doi.org/10.1117/12.912751
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KEYWORDS
Stimulated emission depletion microscopy
Microscopy
Confocal microscopy
Photons
Microscopes
Image resolution
Luminescence
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