Real-time detection of &#945;-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor

Chi-Chieh Huang; Hsin-Feng Hsu; Sz-Hau Chen; Kun-Yu Tsai; Yang-Tung Huang; Chih-Sheng Lin; Shih-Hsin Hsu

doi:10.1117/12.915947

30 January 2012 Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor

Chi-Chieh Huang, Hsin-Feng Hsu, Sz-Hau Chen, Kun-Yu Tsai, Yang-Tung Huang, Chih-Sheng Lin, Shih-Hsin Hsu

Author Affiliations +

Proceedings Volume 8351, Third Asia Pacific Optical Sensors Conference; 83512R (2012) https://doi.org/10.1117/12.915947
Event: Asia Pacific Optical Sensors Conference, 2012, Sydney, Australia

Abstract

αIn this paper, real-time characterization of α-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with α-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for -thrombin detection was achieved.

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Chi-Chieh Huang, Hsin-Feng Hsu, Sz-Hau Chen, Kun-Yu Tsai, Yang-Tung Huang, Chih-Sheng Lin, and Shih-Hsin Hsu "Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor", Proc. SPIE 8351, Third Asia Pacific Optical Sensors Conference, 83512R (30 January 2012); https://doi.org/10.1117/12.915947

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