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In the recent past, there have been enormous efforts to understand effect of drugs on human body. Prior to
understand the effect of drugs on human body most of the experiments are carried out on cells or model organisms. Here
we present our study on the effect of chemotherapeutic drugs on cancer cells and the acetaminophen (APAP) induced
hepatotoxicity in mouse model. Histone deacetylase inhibitors (HDIs) have attracted attention as potential drug
molecules for the treatment of cancer. These are the chemotherapeutic drugs which have indirect mechanistic action
against cancer cells via acting against histone deacetylases (HDAC). It has been known that different HDAC enzymes
are over-expressed in various types of cancers for example; HDAC1 is over expressed in prostate, gastric and breast
carcinomas. Therefore, in order to optimise chemotherapy, it is important to determine the efficacy of various classes of
HDAC inhibitor drugs against variety of over-expressed HDAC enzymes. In the present study, FTIR microspectroscopy
has been employed to predict the acetylation and propionylation brought in by HDIs.
The liver plays an important role in cellular metabolism and is highly susceptible to drug toxicity. APAP which
is an analgesic and antipyretic drug is extensively used for therapeutic purposes and has become the most common cause
of acute liver failure (ALF). In the current study, we have focused to understand APAP induced hepatotoxicity using
FTIR microspectroscopy. In the IR spectrum the bands corresponding to glycogen, ester group and were found to be
suitable markers to predict liver injury at early time point (0.5hr) due to APAP both in tissue and serum in comparison to
standard biochemical assays. Our studies show the potential of FTIR spectroscopy as a rapid, sensitive and non invasive
detection technique for future clinical diagnosis.
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