Accurate and quantitative phase retrieval methods for a series of defocused images with application to in-line Gabor microscopy

Konstantinos Falaggis; Tomasz Kozacki; Michal Jozwik; Malgorzata Kujawinska

doi:10.1117/12.929303

13 September 2012 Accurate and quantitative phase retrieval methods for a series of defocused images with application to in-line Gabor microscopy

Konstantinos Falaggis, Tomasz Kozacki, Michal Jozwik, Malgorzata Kujawinska

Author Affiliations +

Proceedings Volume 8493, Interferometry XVI: Techniques and Analysis; 84930X (2012) https://doi.org/10.1117/12.929303
Event: SPIE Optical Engineering + Applications, 2012, San Diego, California, United States

Abstract

In 1948, Gabor proposed a lens-less in-line holographic microscope where the recorded hologram is a result of interference of both reference wave and the wave diffracted from the object. In this work, an analysis of the hologram formation and sampling issues are presented using the local-spatial frequency distribution at the camera plane. Techniques are presented that enable the reconstruction of the complex object wave at the camera plane from a series of defocused images. It is suggested to use the band-limited angular spectrum method for the back-propagation of the field components, so that an accurate and quantitative estimate of the object-wave at the object plane is obtained. The reconstruction procedure does not rely on the paraxial approximation and is therefore applicable to the case of high NA beams.

Citation Download Citation

Konstantinos Falaggis, Tomasz Kozacki, Michal Jozwik, and Malgorzata Kujawinska "Accurate and quantitative phase retrieval methods for a series of defocused images with application to in-line Gabor microscopy", Proc. SPIE 8493, Interferometry XVI: Techniques and Analysis, 84930X (13 September 2012); https://doi.org/10.1117/12.929303

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