Error analysis of image acquisition by moving objective lens

Hongxia Xie; Hua Chen; Yi Cai; Fengjuan Yang

doi:10.1117/12.2001012

11 December 2012 Error analysis of image acquisition by moving objective lens

Hongxia Xie, Hua Chen, Yi Cai, Fengjuan Yang

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 855329 (2012) https://doi.org/10.1117/12.2001012
Event: Photonics Asia, 2012, Beijing, China

Abstract

In the digital confocal microscopy technology, voltage ceramic is used to drive axial moving of objective lens to collect image slices, which is simple and flexible, easy to control and will get a large precision. But as a result of the movement, the corresponding parameters of the system, even the point spread function changes, which will bring error to the image restoration. According to the principle of optical imaging, the change of the point spread function is quantitative calculated in this paper, through the experiment of recover the image slices collected by moving objective lens and the image slices collected by moving the loading platform, the error is calculated, compared and analyzed. The results can verify the feasibility of moving lens to collect image slices.

Citation Download Citation

Hongxia Xie, Hua Chen, Yi Cai, and Fengjuan Yang "Error analysis of image acquisition by moving objective lens", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855329 (11 December 2012); https://doi.org/10.1117/12.2001012

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KEYWORDS

Objectives

Image restoration

Point spread functions

Error analysis

Digital imaging

Image acquisition

Confocal microscopy

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