Detection of calcium waves in mice heart tissue with multispot two-photon imaging

C. de Mauro; C. A. Cecchetti; D. Alfieri; G. Borile; A. Urbani; M. Mongillo; F. S. Pavone

doi:10.1117/12.2003789

22 February 2013 Detection of calcium waves in mice heart tissue with multispot two-photon imaging

C. de Mauro, C. A. Cecchetti, D. Alfieri, G. Borile, A. Urbani, M. Mongillo, F. S. Pavone

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85882M (2013) https://doi.org/10.1117/12.2003789
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dy- namics at intracellular level. We apply this technique to obtain a time resolution of a few milliseconds, even in full frame images at 512x512 pixels, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue. Further we report on the progress of development of a Random Access microscope for very high speed all optical electrophysiological signal acquisition in cell networks. Our study opens the way to the investigation of arrhythmogenic disease in animal models at cellular level.

Citation Download Citation

C. de Mauro, C. A. Cecchetti, D. Alfieri, G. Borile, A. Urbani, M. Mongillo, and F. S. Pavone "Detection of calcium waves in mice heart tissue with multispot two-photon imaging", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882M (22 February 2013); https://doi.org/10.1117/12.2003789

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