Real-Time Processing Of Interferograms For Monitoring Protein Crystal Growth On The Space Station

A Choudry; N Dupuis

doi:10.1117/12.943399

11 April 1988 Real-Time Processing Of Interferograms For Monitoring Protein Crystal Growth On The Space Station

A Choudry, N Dupuis

Proceedings Volume 0860, Real-Time Image Processing: Concepts and Technologies; (1988) https://doi.org/10.1117/12.943399
Event: 1987 Symposium on the Technologies for Optoelectronics, 1987, Cannes, France

Abstract

We have studied the possibility of using microscopic interferometric techniques to monitor the growth of protein crystals on the Space Station. Nucleation in the solution is a critical phase and needs to be monitored carefully. It involves detecting and localizing nucleation sites in their earliest phase with dimensions of the order of 50-100nm. A comparison with the well-known technique of scattering and microscopic interferometry for monitoring the nucleation was made and the latter seems to be much better suited for this monitoring task. In microscopic interferometry, a detailed map of the solution density is made in the form of an interference pattern. From this pattern one should be able to detect the onset of nucleation. This technique offers several advantages, however, for space applications it is essential that the interferograms must be interpreted in an automated real-time manner. We have used techniques of digital image processing to develop a system for the real-time analysis of microscopic interferograms of nucleation sites during protein crystal growth. Some details of the optical setup and the image processing system along with experimental results will be presented.

Citation Download Citation

A Choudry and N Dupuis "Real-Time Processing Of Interferograms For Monitoring Protein Crystal Growth On The Space Station", Proc. SPIE 0860, Real-Time Image Processing: Concepts and Technologies, (11 April 1988); https://doi.org/10.1117/12.943399

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KEYWORDS

Proteins

Crystals

Interferometry

Microscopes

Crystallography

Microscopy

Real time image processing

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