The enhancement of neuronal cells wound healing with non-contact electric field stimulation by graphene electrodes

Sohee Lee; Chaejeong Heo; Si Young Lee; Young Hee Lee; Minah Suh

doi:10.1117/12.2020770

20 May 2013 The enhancement of neuronal cells wound healing with non-contact electric field stimulation by graphene electrodes

Sohee Lee, Chaejeong Heo, Si Young Lee, Young Hee Lee, Minah Suh

Author Affiliations +

Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 88790X (2013) https://doi.org/10.1117/12.2020770
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

Electrical stimulation affects cellular behaviors including division, migration and wound healing [1-3]. Cellular injury often occurs due to the imbalance of the endogenous electric field [3]. In order to recover from the injury, wound healing process requires various cellular changes such as regeneration, migration, and the enhancement of cytoskeletal proteins and growth factors. In previous reports, a weak non-contact electric field stimulation (nEFS) accelerates the cell migration as well as cell-to-cell coupling between neuronal cell junction which are accompanied by increasing of cytoskeletal proteins [4, 5]. In this paper, we further investigated the wound healing effect of the nEFS in the neuronal cells (SHSY5Y cells) with live cell optical imaging. Cells were cultured over the optically transparent graphenen EF stimulator. Cellular behavioral changes upon nEFS were recorded with live optical imaging during stimulation of 120 minutes. The ability of wound healing was significantly enhanced with the nEFS. In particular, nEFS significantly shorten the duration of wound healing process. Moreover, after treating cells with cytochalasin D, a block polymerization of the actin filaments, the nEFS significantly enhanced wound healing process of cytochalasin D treated neural cells as compared to the control neural cells. This study suggests that nEFS may provide an effective way to control neural cells repairing process from cellular injury. Further mechanism study about the effect of nEFS on the wound healing may shed new light on cellular behavior.

Citation Download Citation

Sohee Lee, Chaejeong Heo, Si Young Lee, Young Hee Lee, and Minah Suh "The enhancement of neuronal cells wound healing with non-contact electric field stimulation by graphene electrodes", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790X (20 May 2013); https://doi.org/10.1117/12.2020770

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Wound healing

Graphene

Electrodes

Proteins

Polymerization

Injuries

In vitro testing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years