Dynamics and morphometric characterization of hippocampus neurons using digital holographic microscopy

Saeid Elkatlawy; María Gomariz; Cristina Soto-Sánchez; Gema Martínez Navarrete; Eduardo Fernández; Antonio Fimia

doi:10.1117/12.2051656

8 May 2014 Dynamics and morphometric characterization of hippocampus neurons using digital holographic microscopy

Saeid Elkatlawy, María Gomariz, Cristina Soto-Sánchez, Gema Martínez Navarrete, Eduardo Fernández, Antonio Fimia

Author Affiliations +

Proceedings Volume 9129, Biophotonics: Photonic Solutions for Better Health Care IV; 91292P (2014) https://doi.org/10.1117/12.2051656
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

In this paper we report on the use of digital holographic microscopy for 3D real time imaging of cultured neurons and neural networks, in vitro. Digital holographic microscopy is employed as an assessment tool to study the biophysical origin of neurodegenerative diseases. Our study consists in the morphological characterization of the axon, dendrites and cell bodies. The average size and thickness of the soma were 21 and 13 μm, respectively. Furthermore, the average size and diameter of some randomly selected neurites were 4.8 and 0.89 μm, respectively. In addition, the spatiotemporal growth process of cellular bodies and extensions was fitted to by a non-linear behavior of the nerve system. Remarkably, this non-linear process represents the relationship between the growth process of cellular body with respect to the axon and dendrites of the neurons.

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Saeid Elkatlawy, María Gomariz, Cristina Soto-Sánchez, Gema Martínez Navarrete, Eduardo Fernández, and Antonio Fimia "Dynamics and morphometric characterization of hippocampus neurons using digital holographic microscopy", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91292P (8 May 2014); https://doi.org/10.1117/12.2051656

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KEYWORDS

Digital holography

Microscopy

Neurons

Holography

Tissues

Microscopes

Holograms

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