Sensitive protein detection using an optical fibre long period grating sensor anchored with silica core gold shell nanoparticles

L. Marques; F. U. Hernandez; S. Korposh; M. Clark; S. Morgan; S. James; R. P. Tatam

doi:10.1117/12.2059196

2 June 2014 Sensitive protein detection using an optical fibre long period grating sensor anchored with silica core gold shell nanoparticles

L. Marques, F. U. Hernandez, S. Korposh, M. Clark, S. Morgan, S. James, R. P. Tatam

Author Affiliations +

Proceedings Volume 9157, 23rd International Conference on Optical Fibre Sensors; 91574S (2014) https://doi.org/10.1117/12.2059196
Event: OFS2014 23rd International Conference on Optical Fiber Sensors, 2014, Santander, Spain

Abstract

An optical fibre long period grating (LPG), modified with a coating of silica gold (SiO₂:Au) core/shell nanoparticles (NPs) deposited using the layer-by-layer (LbL) method, was employed for the development of a bio-sensor. The SiO₂:Au NPs were electrostatically assembled onto the LPG with the aid of a poly(hydrochloride ammonium) (PAH) polycation layer. The LPG sensor operates at the phase matching turning point to provide the highest sensitivity. The SiO₂:Au NPs were modified with biotin, which was used as a ligand for streptavidin (SV) detection. The sensing mechanism is based on the measurement of the refractive index change induced by the binding of the SV to the biotin. The lowest detected concentration of SV was 19 nM using an LPG modified with a 3 layer (PAH/SiO₂:Au) thin film.

Citation Download Citation

L. Marques, F. U. Hernandez, S. Korposh, M. Clark, S. Morgan, S. James, and R. P. Tatam "Sensitive protein detection using an optical fibre long period grating sensor anchored with silica core gold shell nanoparticles", Proc. SPIE 9157, 23rd International Conference on Optical Fibre Sensors, 91574S (2 June 2014); https://doi.org/10.1117/12.2059196

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