Photocatalytic inactivation effect of HMME-TiO2 nanocomposites on SCC cells under visible light

Yulu He; Jianwen Xiong; Cuiping Yao; Jing Wang; Jiansheng Mei; Mengmeng Wang; Zelin Wang; Panting Song; Zhennan Chang; Zhenxi Zhang

doi:10.1117/12.2083110

19 February 2015 Photocatalytic inactivation effect of HMME-TiO₂ nanocomposites on SCC cells under visible light

Yulu He, Jianwen Xiong, Cuiping Yao, Jing Wang, Jiansheng Mei, Mengmeng Wang, Zelin Wang, Panting Song, Zhennan Chang, Zhenxi Zhang

Author Affiliations +

Proceedings Volume 9449, The International Conference on Photonics and Optical Engineering (icPOE 2014); 944910 (2015) https://doi.org/10.1117/12.2083110
Event: The International Conference on Photonics and Optical Engineering and the Annual West China Photonics Conference (icPOE 2014), 2014, Xi'an, China

Abstract

In this paper, HMME-TiO₂nanocomposites was synthesized and characterized through TEM， Uv-vis spectra, Zeta potential, FTIR spectra. The characterization results show that HMME was successfully conjugated onto the surface of TiO₂. It can be seen from the TEM images the average size of HMME-TiO₂ conjugation is nearly spherical and the particle size range from 20 to 28 nm. Compared with HMME, the B bands of HMME-TiO₂ were much broader and lower while in the region of Q bands the absorption peaks of HMME-TiO₂ are higher than that of HMME. Encapsulation efficiency of HMME-loaded TiO₂ was assessed and calculated as 45.46%. FTIR spectra show the bonding between TiO₂ and HMME was through the hydrogen-bonding between COOH and OH bonds. Fluorescence microscope results demonstrated HMME-TiO₂mainly distributed in the membrane and cytoplasm of SCC cells and its best incubation time is six hours. After treated with HMME-TiO₂ plus light irradiation (1.8J/cm² , 632nm), the viability of SCC cells turned to 32.96% is much lower than that treated with HMME plus light irradiation. It can be concluded that the combination of HMME and TiO₂ will enhance the PDT efficiency of HMME. In the process of HMME-TiO₂ mediated PDT, as a kind of photosensitizer HMME can induce the death of SCC cells, meanwhile it can transform electron to the conductive band of TiO₂ stimulating the photocatalytic activity of TiO₂ under visible light. The photocatalytic of TiO₂ can also induce the death of SCC cells. The combination of these two effects lead to more SCC cells died.

Citation Download Citation

Yulu He, Jianwen Xiong, Cuiping Yao, Jing Wang, Jiansheng Mei, Mengmeng Wang, Zelin Wang, Panting Song, Zhennan Chang, and Zhenxi Zhang "Photocatalytic inactivation effect of HMME-TiO₂ nanocomposites on SCC cells under visible light", Proc. SPIE 9449, The International Conference on Photonics and Optical Engineering (icPOE 2014), 944910 (19 February 2015); https://doi.org/10.1117/12.2083110

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available