Flow-cytometric identification of vinegars using a multi-parameter analysis optical detection module

T. Verschooten; H. Ottevaere; M. Vervaeke; J. Van Erps; M. Callewaert; W. De Malsche; H. Thienpont

doi:10.1117/12.2197474

24 September 2015 Flow-cytometric identification of vinegars using a multi-parameter analysis optical detection module

T. Verschooten, H. Ottevaere, M. Vervaeke, J. Van Erps, M. Callewaert, W. De Malsche, H. Thienpont

Proceedings Volume 9628, Optical Systems Design 2015: Optical Fabrication, Testing, and Metrology V; 962822 (2015) https://doi.org/10.1117/12.2197474
Event: SPIE Optical Systems Design, 2015, Jena, Germany

Abstract

We show a proof-of-concept demonstration of a multi-parameter analysis low-cost optical detection system for the flowcytometric identification of vinegars. This multi-parameter analysis system can simultaneously measure laser induced fluorescence, absorption and scattering excited by two time-multiplexed lasers of different wavelengths. To our knowledge no other polymer optofluidic chip based system offers more simultaneous measurements. The design of the optofluidic channels is aimed at countering the effects that viscous fingering, air bubbles, and emulsion samples can have on the correct operation of such a detection system. Unpredictable variations in viscosity and refractive index of the channel content can be turned into a source of information. The sample is excited by two laser diodes that are driven by custom made low-cost laser drivers. The optofluidic chip is built to be robust and easy to handle and is reproducible using hot embossing. We show a custom optomechanical holder for the optofluidic chip that ensures correct alignment and automatic connection to the external fluidic system. We show an experiment in which 92 samples of vinegar are measured. We are able to identify 9 different kinds of vinegar with an accuracy of 94%. Thus we show an alternative approach to the classic optical spectroscopy solution at a lowered. Furthermore, we have shown the possibility of predicting the viscosity and turbidity of vinegars with a goodness-of-fit R² over 0.947.

Citation Download Citation

T. Verschooten, H. Ottevaere, M. Vervaeke, J. Van Erps, M. Callewaert, W. De Malsche, and H. Thienpont "Flow-cytometric identification of vinegars using a multi-parameter analysis optical detection module", Proc. SPIE 9628, Optical Systems Design 2015: Optical Fabrication, Testing, and Metrology V, 962822 (24 September 2015); https://doi.org/10.1117/12.2197474

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Laser scattering

Principal component analysis

Absorption

Scattering

Signal detection

Semiconductor lasers

Sensors

Show All Keywords

Keywords/Phrases

Search In:

Publication Years