Autofluorescence lifetime imaging of macrophage activation in vivo

Veronika Miskolci; Kelsey Tweed; Michael Lasarev; Anna Huttenlocher; Melissa Skala

doi:10.1117/12.2608349

7 March 2022 Autofluorescence lifetime imaging of macrophage activation in vivo

Veronika Miskolci, Kelsey Tweed, Michael Lasarev, Anna Huttenlocher, Melissa Skala

Proceedings Volume PC11961, Biophotonics and Immune Responses XVII; PC1196109 (2022) https://doi.org/10.1117/12.2608349
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Metabolic control of immune cell function has emerged as a key regulatory mechanism in immunity. Current studies rely mainly on in vitro and ex vivo approaches to characterize the intracellular metabolism of immune cells. Here, we show that fluorescence lifetime imaging microscopy of metabolic coenzymes, NAD(P)H and FAD, captures dynamic changes in the metabolic activity of macrophages during diverse inflammatory responses in their native interstitial microenvironments. This imaging-based approach also provides single-cell resolution, in contrast to bulk measurements provided by traditional biochemical assays, making it a potentially valuable tool in the field of immunometabolism.

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Veronika Miskolci, Kelsey Tweed, Michael Lasarev, Anna Huttenlocher, and Melissa Skala "Autofluorescence lifetime imaging of macrophage activation in vivo", Proc. SPIE PC11961, Biophotonics and Immune Responses XVII, PC1196109 (7 March 2022); https://doi.org/10.1117/12.2608349

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KEYWORDS

In vivo imaging

Fluorescence lifetime imaging

Inflammation

Mode conditioning cables

Tissues

Genetics

In vitro testing

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