Video-rate green three-photon fluorescence microscopy based on a Cr:forsterite laser oscillator

Lu-Ting Chou; Hao-Hsuan Hung; Wei-Zong Line; Anatoly A. Ivanov; Shih-Hsuan Chia

doi:10.1117/12.2609610

7 March 2022 Video-rate green three-photon fluorescence microscopy based on a Cr:forsterite laser oscillator

Lu-Ting Chou, Hao-Hsuan Hung, Wei-Zong Line, Anatoly A. Ivanov, Shih-Hsuan Chia

Author Affiliations +

Proceedings Volume PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII; PC119650W (2022) https://doi.org/10.1117/12.2609610
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

We have realized video-rate three-photon fluorescence microscopy based on a simple 1320-nm femtosecond source, which is driven by a 24-MHz Cr:forsterite oscillator and fiber-optic nonlinear conversion. The development aims to find a robust pumping source for deep-tissue GFP imaging. We optimized the laser oscillator to deliver 40-nJ output pulse energy at around 1260 nm, and the matching of the laser spectrum and GFP excitation can be realized by precisely control the interplay between self-phase modulation and fiber dispersion. We have obtained clear video-rate three-photon imaging from fluorescent beads, and further optimization leading to deep-tissue GFP imaging will be discussed.

Conference Presentation

Citation Download Citation

Lu-Ting Chou, Hao-Hsuan Hung, Wei-Zong Line, Anatoly A. Ivanov, and Shih-Hsuan Chia "Video-rate green three-photon fluorescence microscopy based on a Cr:forsterite laser oscillator", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC119650W (7 March 2022); https://doi.org/10.1117/12.2609610

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