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2 March 2022 Metabolic imaging using the phasor approach to FLIM and tracking phenotypic change of mitochondria in cancer cells with Mitometer
Michelle Digman
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Proceedings Volume PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV; PC1196705 (2022) https://doi.org/10.1117/12.2619390
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
The hallmark of metabolic alteration of increase glycolysis, i.e. Warburg effect, in cancer cells together with atypical extracellular matrix structure may be responsible for tumor cell aggressiveness and drug resistance. Here we apply the phasor approach technique in fluorescence lifetime imaging microscopy (FLIM) as a novel method to measure metabolic alteration as a function of ECM mechanics. We imaged and compared triple-negative breast cancer (TNBC) cells to non-cancerous cells on various ECM stiffness. Dysregulation of mitochondrial motion may contribute to the fueling of bioenergy demands in metastatic cancer. To measure mitochondria motion and analyze their fusion and fission events, we developed a new algorithm called “mitometer” that is unbiased, and allows for automated segmentation and tracking of mitochondria in live cell 2D and 3D time-lapse images.
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Michelle Digman "Metabolic imaging using the phasor approach to FLIM and tracking phenotypic change of mitochondria in cancer cells with Mitometer", Proc. SPIE PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV, PC1196705 (2 March 2022); https://doi.org/10.1117/12.2619390
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KEYWORDS
Cancer
Fluorescence lifetime imaging
Breast cancer
Tumors
Algorithm development
Breast
Detection and tracking algorithms
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