2 March 2022Label-free three dimensional optical coherence tomography (OCT-) based imaging method for drug response evaluation of human derived tumor spheroids
1Institute of Applied Physics, Univ. of Tsukuba (Japan) 2Damietta Univ. (Egypt) 3Sky Technology Inc. (Japan) 4Univ. of Tsukuba (Japan) 5Computational Optics Group, Institute of Applied Physics, Univ. of Tsukuba (Japan)
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We present a non-invasive (label-free) and 3-D method for anti-cancer drug response evaluation of human originated tumor spheroids. Our method is a combination of an OCT microscope and a statistical analysis framework of the OCT signal temporal fluctuations induced by the intra-cellular motions. This method allows the visualization of the 3-D metabolic activity of tumor spheroids and its alteration induced by the drug applications. Two human originated tumor spheroid’s drug responses were evaluated including breast adenocarcinoma (MCF7 cell-line) and colorectal carcinoma (HT-29 cell-line). The drug response was not only visualized as an images, but also quantitative analysis has been performed.
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Ibrahim G. Abd El-Sadek, Arata Miyazawa, Tomoko Mori, Larina Shen, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, Yoshiaki Yasuno, "Label-free three dimensional optical coherence tomography (OCT-) based imaging method for drug response evaluation of human derived tumor spheroids," Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720N (2 March 2022); https://doi.org/10.1117/12.2608156