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2 March 2022 High speed spatial frequency modulation nonlinear spectroscopic microscopy
Randy A. Bartels, Sandro Heuke, Jeff Field, Jeff Squier, Anne Sentenac, Hervé Rigneault
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Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730R (2022) https://doi.org/10.1117/12.2613516
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Raman microscopy is a powerful mode of label free nonlinear optical microscopy that is hampered by weak cross-sections, leading to slow imaging. We will discuss two advances in coherent Raman microscopy: 1) high speed coherent anti-Stokes Raman scattering microscopy imaging based on spatial frequency modulation imagining (SPIFI) where a structured line focus is used to image is used to image specimens with a single pixel detector. 2) Doppler Raman microscopy that exploits the extremely low timing jitter of modelocked lasers for ultrasensitive Raman spectroscopy and microscopy.
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Randy A. Bartels, Sandro Heuke, Jeff Field, Jeff Squier, Anne Sentenac, and Hervé Rigneault "High speed spatial frequency modulation nonlinear spectroscopic microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730R (2 March 2022); https://doi.org/10.1117/12.2613516
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KEYWORDS
Microscopy
Modulation
Spatial frequencies
Raman spectroscopy
Spectroscopy
CARS tomography
Doppler effect
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