Toward whole brain label-free molecular imaging with single-cell resolution sing ultra-broadband multiplex CARS microspectroscopy

Yusuke Murakami; Shinichi Miyazaki; Damien Boildieu; Zakaniaina Rajaofara; David Helbert; Amandine Magnaudeix; Philippe Carré; Philippe Leproux; Sakiko Honjoh; Hideaki Kano

doi:10.1117/12.2626342

2 March 2022 Toward whole brain label-free molecular imaging with single-cell resolution sing ultra-broadband multiplex CARS microspectroscopy

Yusuke Murakami, Shinichi Miyazaki, Damien Boildieu, Zakaniaina Rajaofara, David Helbert, Amandine Magnaudeix, Philippe Carré, Philippe Leproux, Sakiko Honjoh, Hideaki Kano

Author Affiliations +

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119731B (2022) https://doi.org/10.1117/12.2626342
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Mapping the distribution of chemical molecules throughout a brain is helpful for neuroscience research. We have applied an ultra-broadband multiplex CARS spectroscopic imaging system to construct a whole-brain label-free molecular map in macro and micro scales. We could precisely visualize lipids distributed to white matter, rich in neuronal fibers. Our microscale measurements figured out that cells within the hippocampus and cerebral cortex could be divided into lipid-rich and water-rich cells. Moreover, we applied multivariate curve decomposition analysis for our spectrum and recapitulated the results. Our imaging and analysis techniques will lead to the molecular brain atlas with single-cell resolution.

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Yusuke Murakami, Shinichi Miyazaki, Damien Boildieu, Zakaniaina Rajaofara, David Helbert, Amandine Magnaudeix, Philippe Carré, Philippe Leproux, Sakiko Honjoh, and Hideaki Kano "Toward whole brain label-free molecular imaging with single-cell resolution sing ultra-broadband multiplex CARS microspectroscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119731B (2 March 2022); https://doi.org/10.1117/12.2626342

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