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9 March 2022 3D Phase and fluorescence microscopy with scattering samples
Laura Waller
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Proceedings Volume PC12017, Complex Light and Optical Forces XVI; PC120170D (2022) https://doi.org/10.1117/12.2611445
Event: SPIE OPTO, 2022, San Francisco, California, United States
Abstract
This talk will describe new microscopy methods that use computational imaging to enable 3D phase (refractive index) and 3D fluorescence measurements in samples that are thick or incur multiple scattering, such as embryos or whole organisms. We use image reconstruction algorithms that are based on large-scale nonlinear non-convex optimization to model the multiple scattering effects of light passing through the sample, and discuss end-to-end learned designs for optimizing the hardware configurations.
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Laura Waller "3D Phase and fluorescence microscopy with scattering samples", Proc. SPIE PC12017, Complex Light and Optical Forces XVI, PC120170D (9 March 2022); https://doi.org/10.1117/12.2611445
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KEYWORDS
3D metrology
Luminescence
Microscopy
Scattering
3D image processing
Multiple scattering
Organisms
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