CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC12198 > Article
Presentation
3 October 2022 Thermal noise imaging probes the 3D structure and dynamics of microtubule networks with 4 nm precision
Michael Himmelsbach, Jordan Zesch, Ernst-Ludwig Florin
Author Affiliations +
Proceedings Volume PC12198, Optical Trapping and Optical Micromanipulation XIX; PC1219802 (2022) https://doi.org/10.1117/12.2635909
Event: SPIE Nanoscience + Engineering, 2022, San Diego, California, United States
Abstract
The mechanics of filament networks depend on both the individual filament properties and the network architecture. Despite the importance of filament networks in biology, there exists no technique which can precisely localize filaments and cross-links in three dimensions and simultaneously resolve their dynamics. Thermal noise imaging is a three-dimensional scanning probe technique that utilizes the confined thermal motion of an optically trapped nanoparticle to noninvasively probe the sample. Here, we apply the technique to a stably crosslinked network of microtubules. The filament axes are localized with a precision of 4nm. Analysis also reveals fluctuations of individual filaments and properties of cross-links.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Michael Himmelsbach, Jordan Zesch, and Ernst-Ludwig Florin "Thermal noise imaging probes the 3D structure and dynamics of microtubule networks with 4 nm precision", Proc. SPIE PC12198, Optical Trapping and Optical Micromanipulation XIX, PC1219802 (3 October 2022); https://doi.org/10.1117/12.2635909
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
3D image processing
Stereoscopy
Network architectures
Cytoskeletons
Diffraction
Mechanics
Nanoparticles
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top