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17 March 2023 Multi-functionalities of nanodiamond as a neuritogenic promoter and dSTORM super-resolution imaging probe
Jaeheung Kim, Moon Sung Kang, Dong-Wook Han, Chang-Seok Kim
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Proceedings Volume PC12365, Neural Imaging and Sensing 2023; PC123650H (2023) https://doi.org/10.1117/12.2649775
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We suggest the multi-functions of nanodiamond (ND) as a neuritogenesis promoter and super-resolution imaging probe. Owing to the photoblinking property of ND, bioimages were visualized in super-resolution using the direct stochastic optical reconstruction microscopy process, which resolved the ambiguity in the distinction of nearby particles. In this study, NDs were endocytosed by HT-22 hippocampal neuronal cells and promoted a spontaneous neuritogenesis without any differentiation factor, which was visualized by NDs as super-resolution imaging probes without toxicity. In conclusion, it is suggested that ND has excellent neuritogenic capability and potential as a super-resolution neural imaging probe with its outstanding biocompatibility.
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Jaeheung Kim, Moon Sung Kang, Dong-Wook Han, and Chang-Seok Kim "Multi-functionalities of nanodiamond as a neuritogenic promoter and dSTORM super-resolution imaging probe", Proc. SPIE PC12365, Neural Imaging and Sensing 2023, PC123650H (17 March 2023); https://doi.org/10.1117/12.2649775
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KEYWORDS
Super resolution
Neodymium
Image processing
In vitro testing
Microscopy
Particles
Stochastic processes
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