LEAD microscopy performing at 100’s kHz frames per second for nonlinear imaging and 3D-imaging flow cytometry (Conference Presentation)

Adela Ben-Yakar

doi:10.1117/12.2654828

15 March 2023 LEAD microscopy performing at 100’s kHz frames per second for nonlinear imaging and 3D-imaging flow cytometry (Conference Presentation)

Adela Ben-Yakar

Author Affiliations +

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840A (2023) https://doi.org/10.1117/12.2654828
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

I will present a new fluorescence imaging method called LEAD (line excitation array detection) microscopy, capable of providing 0.8 million frames per second. This method performs line-scanning of excitation laser beam using a chirped signal-driven longitudinal acousto-optic deflector to create a virtual light-sheet, and images the field-of-view with a linear photomultiplier tube array to generate a 66×14 pixel frame each scan cycle. I will present an implementation of the LEAD microscopy as a blur-free 3D imaging flow cytometer with 3.5-micron resolution and signal-to-background ratios >200. I will also present its conceptual implementation as an ultrafast two-photon LEAD microscopy (2p-LEAD).

Conference Presentation

Citation Download Citation

Adela Ben-Yakar "LEAD microscopy performing at 100’s kHz frames per second for nonlinear imaging and 3D-imaging flow cytometry (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840A (15 March 2023); https://doi.org/10.1117/12.2654828

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