Localization based super-resolution fluorescence imaging of amyloid-β aggregates in mouse brain (Conference Presentation)

Yuki Shimaoka; Daiki Hara; Aman Shekh; Masaya Okada; Shigeki Iwanaga

doi:10.1117/12.2649873

15 March 2023 Localization based super-resolution fluorescence imaging of amyloid-β aggregates in mouse brain (Conference Presentation)

Yuki Shimaoka, Daiki Hara, Aman Shekh, Masaya Okada, Shigeki Iwanaga

Author Affiliations +

Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC123860E (2023) https://doi.org/10.1117/12.2649873
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Abnormal accumulation of amyloid-β (Aβ) plaques in the brain is one of the major characteristics of Alzheimer’s disease and there is a possibility that the degree of plaque toxicity is related to the distribution of nanoscale oligomeric aggregates in plaques. Here, by super-resolution fluorescence imaging, we visualized that anti-Aβ oligomer antibodies localized differently to plaques compared to anti-Aβ monomer antibodies and characteristic plaque shapes in brain tissues were classified. These differences in nanoscale distribution were hard to be discerned by conventional fluorescence imaging, implying that super-resolution imaging has the potential to reveal the detailed features of oligomeric aggregates in plaques.

Conference Presentation

Citation Download Citation

Yuki Shimaoka, Daiki Hara, Aman Shekh, Masaya Okada, and Shigeki Iwanaga "Localization based super-resolution fluorescence imaging of amyloid-β aggregates in mouse brain (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC123860E (15 March 2023); https://doi.org/10.1117/12.2649873

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