Quantitative phase microscopy for biomaterial and device characterization

Peter T. C. So

doi:10.1117/12.2676493

12 August 2023 Quantitative phase microscopy for biomaterial and device characterization

Peter T. C. So

Proceedings Volume PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; PC1262203 (2023) https://doi.org/10.1117/12.2676493
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

We have developed different classes of quantitative phase microscope for applications ranges from material inspection to biomedical assays in cells and tissues. Here we focus on quantifying the conformations of many driven and active nematic fluids exhibit complex microstructures. We explore the structures emerging in a pressure-driven nematic lyotropic chromonic liquid crystal in a microfluidic channel. We show that twist-type topological defects spontaneously emerge under flow. Our single-shot quantitative polarization imaging method allows us to quantify the fluctuations of these defects, which we show to reflect the tumbling character of the liquid crystal. We report how the defect size is governed by the balance between nucleation and annihilation forces, a balance that can be tuned by the flow rate. Such control over the microstructure opens pathways for using these nematic materials in optical devices and to control assembly of biological systems. If time permits, we will further present a novel nanofabrication technology for creating novel meta-optical components with high refractive index contrast based on Implosion Fabrication and will describe the applications of quantitative phase imaging for manufacturing process control.

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Citation Download Citation

Peter T. C. So "Quantitative phase microscopy for biomaterial and device characterization", Proc. SPIE PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, PC1262203 (12 August 2023); https://doi.org/10.1117/12.2676493

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KEYWORDS

Microscopy

Control systems

Liquid crystals

Microfluidics

Phase imaging

Polarization imaging

Process control

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