Improved TIRF imaging through resonant dielectric multilayer optimization

Anita Mouttou; Yousra Toumi; Fabien Lemarchand; Cihan Koc; Antonin Moreau; Delphine Muriaux; Guillaume Demésy; Julien Lumeau; Cyril Favard; Aude L. Lereu

doi:10.1117/12.2676378

4 October 2023 Improved TIRF imaging through resonant dielectric multilayer optimization

Anita Mouttou, Yousra Toumi, Fabien Lemarchand, Cihan Koc, Antonin Moreau, Delphine Muriaux, Guillaume Demésy, Julien Lumeau, Cyril Favard, Aude L. Lereu

Author Affiliations +

Proceedings Volume PC12647, Active Photonic Platforms (APP) 2023; PC126471I (2023) https://doi.org/10.1117/12.2676378
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

We present the optimization of dielectric multilayers (DM) for improved total internal reflection fluorescence sensitivity. The desired resonances require a design that achieves a field enhancement and an angular tolerance of the resonance in the order of the illumination divergence. We studied the effect of the imaginary part of the refractive index k of the DM top layer on the fluorescence enhancement. We established a protocol to fabricate a single layer with a controlled k and fabricated three structures of various k. We tested the concept on fluorescent beads and observed a good agreement with the predicted fluorescence enhancement.

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Anita Mouttou, Yousra Toumi, Fabien Lemarchand, Cihan Koc, Antonin Moreau, Delphine Muriaux, Guillaume Demésy, Julien Lumeau, Cyril Favard, and Aude L. Lereu "Improved TIRF imaging through resonant dielectric multilayer optimization", Proc. SPIE PC12647, Active Photonic Platforms (APP) 2023, PC126471I (4 October 2023); https://doi.org/10.1117/12.2676378

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