Variable metalens-based optical sectioning fluorescence microscopy with HiLo algorithm

Cheng Hung Chu; Yu-Hsin Chia; Mu Ku Chen; Sunil Vyas; Din Ping Tsai; Yuan Luo

doi:10.1117/12.2676659

5 October 2023 Variable metalens-based optical sectioning fluorescence microscopy with HiLo algorithm

Cheng Hung Chu, Yu-Hsin Chia, Mu Ku Chen, Sunil Vyas, Din Ping Tsai, Yuan Luo

Proceedings Volume PC12648, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XXI; PC126480L (2023) https://doi.org/10.1117/12.2676659
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

We present the use of Moiré metalens in fluorescence microscopy system combining with telecentric system and HiLo algorithm for bio-imaging. The Moiré metalens is composed of paired phase metasurfaces. The system is capable of tuning the focal length from 10 mm to 125 mm by tuning the relative angle between the paired metasurfaces without axial displacement. In addition, the speckle illumination HiLo microscopy is used to mitigate the out-of-focus light scattering, the background noise. The labeled beads and ex vivo mouse intestinal tissue samples are imaged to show the optical sectioning capacity of our system. It is anticipated that the proposed design of varifocal metalens would have significant applications in fluorescence microscopy and endoscopy.

Conference Presentation

Citation Download Citation

Cheng Hung Chu, Yu-Hsin Chia, Mu Ku Chen, Sunil Vyas, Din Ping Tsai, and Yuan Luo "Variable metalens-based optical sectioning fluorescence microscopy with HiLo algorithm", Proc. SPIE PC12648, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XXI, PC126480L (5 October 2023); https://doi.org/10.1117/12.2676659

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