Dynamic optical coherence microscopy integrated with confocal fluorescence microscopy to study cell migration in 3D cancer models

Alireza Mowla; Philip Wijesinghe; Jiayue Li; Matt S. Hepburn; Sebastian Amos; Danielle Vahala; Liisa M. Hirvonen; Samuel Maher; Yu Suk Choi; Brendan F. Kennedy

doi:10.1117/12.3005748

13 March 2024 Dynamic optical coherence microscopy integrated with confocal fluorescence microscopy to study cell migration in 3D cancer models

Alireza Mowla, Philip Wijesinghe, Jiayue Li, Matt S. Hepburn, Sebastian Amos, Danielle Vahala, Liisa M. Hirvonen, Samuel Maher, Yu Suk Choi, Brendan F. Kennedy

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Proceedings Volume PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; PC128302J (2024) https://doi.org/10.1117/12.3005748
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Multicellular tumour spheroids have recently become important tools to investigate different stages of cancer development due to their 3D nature. We propose dynamic optical coherence microscopy (OCM) as a label-free low coherence interferometric technique for 3D characterization of morphology and cell motility during cell migration in multicellular cancer cell spheroids. We integrate dynamic OCM with confocal fluorescence microscopy (CFM) to validate and co-register the subcellular-scale endogenous contrast generated by dynamic OCM signal with sub-cellular features such as cell nucleus and membrane. We apply dynamic OCM integrated with CFM to scan metastatic and non-metastatic breast cancer cell spheroids embedded in gelatin-methacryloyl (GelMA) hydrogel and demonstrate that dynamic OCM provides high-contrast morphological imaging equivalent to that of confocal fluorescence in cancer cell spheroids. We use dynamic OCM to visualize different phases of cell migration such as invadopodia formation, cells breaking off from the primary tumour model, and migrating cells presenting a spindle-like shape, and to characterize cell motility at different stages.

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Alireza Mowla, Philip Wijesinghe, Jiayue Li, Matt S. Hepburn, Sebastian Amos, Danielle Vahala, Liisa M. Hirvonen, Samuel Maher, Yu Suk Choi, and Brendan F. Kennedy "Dynamic optical coherence microscopy integrated with confocal fluorescence microscopy to study cell migration in 3D cancer models", Proc. SPIE PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, PC128302J (13 March 2024); https://doi.org/10.1117/12.3005748

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KEYWORDS

3D modeling

Cancer

Confocal microscopy

Fluorescence microscopy

Optical coherence microscopy

Tumor growth modeling

Data acquisition

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