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13 March 2024 Comparing deep ultraviolet transmission microscopy and ultraviolet photoacoustic remote sensing microscopy for thin-tissue histology
Brendyn D. Cikaluk, Mohammad H. Masoumi, Nathaniel J. M. Haven, Matthew T. Martell, Brendon S. Restall, Roger J. Zemp
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Proceedings Volume PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024; PC128421N (2024) https://doi.org/10.1117/12.3002868
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Ultraviolet photacoustic remote sensing (UV-PARS) microscopy is a non-contact, label-free imaging modality which has demonstrated the ability to generate virtual histology images that show good concordance to gold-standard hematoxylin and eosin (H&E) stains of fixed tissue sections. However, UV-PARS microscopy requires time-consuming and complex coalignment of multiple beams for imaging. In this work, we demonstrate UV transmittance and scattering microscopy for virtual histology and compare with UV-PARS microscopy. Maximally realistic virtual histology images are generated for both UV-PARS and UV-transmittance microscopy techniques using a cycle-consistent generative adversarial network (CycleGAN) and compared to one another alongside the gold-standard brightfield microscopy of H&E stained fixed tissues.
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Brendyn D. Cikaluk, Mohammad H. Masoumi, Nathaniel J. M. Haven, Matthew T. Martell, Brendon S. Restall, and Roger J. Zemp "Comparing deep ultraviolet transmission microscopy and ultraviolet photoacoustic remote sensing microscopy for thin-tissue histology", Proc. SPIE PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024, PC128421N (13 March 2024); https://doi.org/10.1117/12.3002868
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KEYWORDS
Microscopy
Ultraviolet radiation
Photoacoustic spectroscopy
Remote sensing
Tissues
Transmittance
Diagnostics
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