Towards in-vivo, non-invasive, label-free, real-time 3D H&E imaging for clinical applications via 3D phase contrast from quantitative oblique back illumination microcopy (qOBM)

Francisco E. Robles; Richard Levenson; Tanishq M. Abraham; Paloma Casteleiro Costa; Zhe Guang; Caroline E. Serafini

doi:10.1117/12.3008577

13 March 2024 Towards in-vivo, non-invasive, label-free, real-time 3D H&E imaging for clinical applications via qOBM

Francisco E. Robles, Richard Levenson, Tanishq M. Abraham, Paloma Casteleiro Costa, Zhe Guang, Caroline E. Serafini

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520Z (2024) https://doi.org/10.1117/12.3008577
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Quantitative oblique-back-illumination microscopy (qOBM) enables quantitative phase imaging (QPI) with epi-illumination, and thus permits the use of phase contrast in applications that were previously out-of-reach for QPI, including clinical medicine. Here, I will discuss our latest efforts to apply qOBM for clinical applications, specifically tissue imaging for non-invasive diagnostics and image guided therapy. Our approach uses an unsupervised cycle generative adversarial networks to translate 3D phase images of thick fresh tissues to appear like H&E-stained tissue sections. This work paves the way for non-invasive, label-free, real-time 3D H&E imaging which can be transformative for disease detection and guided therapy.

Conference Presentation

Citation Download Citation

Francisco E. Robles, Richard Levenson, Tanishq M. Abraham, Paloma Casteleiro Costa, Zhe Guang, and Caroline E. Serafini "Towards in-vivo, non-invasive, label-free, real-time 3D H&E imaging for clinical applications via qOBM", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520Z (13 March 2024); https://doi.org/10.1117/12.3008577

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

3D image processing

Biological imaging

Biomedical applications

Light sources and illumination

Real time imaging

Stereoscopy

Tissues

Show All Keywords

Keywords/Phrases

Search In:

Publication Years