High-throughput and high-resolution single-cell biophysical fractometry for functional cell morphological profiling

Ziqi Zhang; Kevin Tsia; Kelvin C.M. Lee; Dickson M. D. Siu; Michelle C. K. Lo; Edmund Y. Lam

doi:10.1117/12.3008474

13 March 2024 High-throughput and high-resolution single-cell biophysical fractometry for functional cell morphological profiling

Ziqi Zhang, Kevin Tsia, Kelvin C.M. Lee, Dickson M. D. Siu, Michelle C. K. Lo, Edmund Y. Lam

Author Affiliations +

Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC128530R (2024) https://doi.org/10.1117/12.3008474
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We developed a large-scale morphological profiling approach that reads out a multitude of high-resolution biophysical fractal properties of single cells, based on our high-throughput quantitative phase imaging (QPI) platform (at 10,000 cells/sec). We showed that the single-cell morphological profile constructed by the sub-cellular fractal characteristics could be harnessed to implicate cell types and states in the context of identifying lung cancer cell line subtypes, assessing cell morphology in response to drug treatment, and tracking cell cycle progression.

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Citation Download Citation

Ziqi Zhang, Kevin Tsia, Kelvin C.M. Lee, Dickson M. D. Siu, Michelle C. K. Lo, and Edmund Y. Lam "High-throughput and high-resolution single-cell biophysical fractometry for functional cell morphological profiling", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530R (13 March 2024); https://doi.org/10.1117/12.3008474

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