Two orders of magnitude faster tuning and about 10x faster image acquisition in SRS microscopy including the fingerprint region of relevant biological samples

Ingo Rimke; Gero Stibenz; Stefan Popien; Peter Trabs; Lenny Reinkensmeier; Rene Siegmund; Alexander Egner; Sandro Heuke

doi:10.1117/12.3000203

13 March 2024 Two orders of magnitude faster tuning and about 10x faster image acquisition in SRS microscopy including the fingerprint region of relevant biological samples

Ingo Rimke, Gero Stibenz, Stefan Popien, Peter Trabs, Lenny Reinkensmeier, Rene Siegmund, Alexander Egner, Sandro Heuke

Author Affiliations +

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128551B (2024) https://doi.org/10.1117/12.3000203
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We have developed a new shot noise limited light source for Coherent Raman microscopy and other nonlinear microscopy modalities such as SHG and multiphoton fluorescence. Compared to the market leading SRS light source it achieves 100x faster tuning for random wavelength access including power control and dispersion compensation. Further switching from 80 to 40MHz repetition rate with subharmonic modulation frequency of 20MHz reduces the pixel dwell time in theory by a factor of 8 for the same signal to noise ratio. We will present a comparison to the prevailing SRS light source along with fingerprint images of relevant biological samples.

Conference Presentation

Citation Download Citation

Ingo Rimke, Gero Stibenz, Stefan Popien, Peter Trabs, Lenny Reinkensmeier, Rene Siegmund, Alexander Egner, and Sandro Heuke "Two orders of magnitude faster tuning and about 10x faster image acquisition in SRS microscopy including the fingerprint region of relevant biological samples", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128551B (13 March 2024); https://doi.org/10.1117/12.3000203

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