Effects of fat concentration and milk fat globule size distribution on angular light scattering in human milk

Wietske Verveld; David Thompson; Johanna R. de Wolf; Hanne R. van de Glind; Nienke Bosschaart

doi:10.1117/12.2689595

13 March 2024 Effects of fat concentration and milk fat globule size distribution on angular light scattering in human milk

Wietske Verveld, David Thompson, Johanna R. de Wolf, Hanne R. van de Glind, Nienke Bosschaart

Author Affiliations +

Proceedings Volume PC12856, Biomedical Applications of Light Scattering XIV; PC128560I (2024) https://doi.org/10.1117/12.2689595
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Measurements of human milk fat content are essential for lactation care and research. We propose to quantify milk fat in non-homogenized human milk based on angular light scattering. Therefore, we measured the angular scattering profiles of milk from five donors using a goniometric light scattering setup. We also measured the milk fat globule (MFG) size distributions with 3D confocal laser scanning microscopy and use this as input in a Monte Carlo simulation. Both experimental and simulated angular scattering profiles are strongly dependent on fat concentration. The effect of the MFG size distribution on the scattering profiles will be discussed.

Citation Download Citation

Wietske Verveld, David Thompson, Johanna R. de Wolf, Hanne R. van de Glind, and Nienke Bosschaart "Effects of fat concentration and milk fat globule size distribution on angular light scattering in human milk", Proc. SPIE PC12856, Biomedical Applications of Light Scattering XIV, PC128560I (13 March 2024); https://doi.org/10.1117/12.2689595

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