Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy

Qing Xia; Zhongyue Guo; Haonan Zong; Scott Seitz; Celalettin Yurdakul; M. Selim Ünlü; Le Wang; John H. Connor; Ji-Xin Cheng

doi:10.1117/12.3000715

13 March 2024 Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy

Qing Xia, Zhongyue Guo, Haonan Zong, Scott Seitz, Celalettin Yurdakul, M. Selim Ünlü, Le Wang, John H. Connor, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; PC128620Q (2024) https://doi.org/10.1117/12.3000715
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Label-free spectroscopic detection of single viruses provides component analysis of virus strains. Current methods suffer from low throughput and weak signal contrast of individual virions. Here, we report a widefield interferometric defocus-enhanced mid-infrared photothermal (WIDE-MIP) microscopy for high-throughput fingerprinting of single viruses. WIDE-MIP not only reveals the contents of viral proteins and nucleic acids in single DNA vaccinia viruses and RNA vesicular stomatitis viruses, but also uncovers an enriched β sheet components in DNA varicella-zoster virus proteins. Different nucleic acids signatures of thymine and uracil residue vibrations are also obtained to differentiate DNA and RNA viruses.

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Qing Xia, Zhongyue Guo, Haonan Zong, Scott Seitz, Celalettin Yurdakul, M. Selim Ünlü, Le Wang, John H. Connor, and Ji-Xin Cheng "Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy", Proc. SPIE PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, PC128620Q (13 March 2024); https://doi.org/10.1117/12.3000715

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