Nanophotonic-enabled upconverting nanoparticle immunoassay for detecting ultralow concentrations of biomarkers in blood

Chia-Chen Hsu; Yen-Ta Tseng; Yu-Chung Chiu; Van-Dai Pham; Wen-Hsuan Wu; Thanh Thu Le-Vu; Chih-Hsien Wang; Shiao-Wei Kuo; W. Y. M. Chan; Chun-Hung Lin; Szu-Chin Li; Yi-Da Li; Hung-Chih Kan; Jiunn-Yuan Lin; Lai-Kwan Chau

doi:10.1117/12.3026956

4 October 2024 Nanophotonic-enabled upconverting nanoparticle immunoassay for detecting ultralow concentrations of biomarkers in blood

Chia-Chen Hsu, Yen-Ta Tseng, Yu-Chung Chiu, Van-Dai Pham, Wen-Hsuan Wu, Thanh Thu Le-Vu, Chih-Hsien Wang, Shiao-Wei Kuo, W. Y. M. Chan, Chun-Hung Lin, Szu-Chin Li, Yi-Da Li, Hung-Chih Kan, Jiunn-Yuan Lin, Lai-Kwan Chau

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Proceedings Volume PC13116, Nanoengineering: Fabrication, Properties, Optics, Thin Films, and Devices XXI; PC1311601 (2024) https://doi.org/10.1117/12.3026956
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Abstract

Nanophotonic structures can enhance light-mater interaction at nanoscales helping to improve the detection sensitivity of biosensors. In this work, we present a sandwich-type immunosensor by combining a nanophotonic resonant waveguide grating (RWG) structure and upconverting nanoparticles (UCNPs). UCNPs are used to label a target biomarker captured by capture antibody molecules immobilized on the surface of the RWG structure, which is used to enhance the upconversion fluorescence (UCF) of UCNPs through excitation resonance. The immunosensor has an extremely low limit of detection (LOD) in sub-fg/mL level and a detection range of six orders of magnitude and can be used to detect a variety of biomarkers such as cardiac troponin I, tau protein and phosphated-tau protein, etc. The LOD of the immunosensor is greatly reduced due to the increased UCF of UCNPs and the reduction of nonspecific adsorption of detection antibody-conjugated UCNPs on the RWG substrate surface.

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Chia-Chen Hsu, Yen-Ta Tseng, Yu-Chung Chiu, Van-Dai Pham, Wen-Hsuan Wu, Thanh Thu Le-Vu, Chih-Hsien Wang, Shiao-Wei Kuo, W. Y. M. Chan, Chun-Hung Lin, Szu-Chin Li, Yi-Da Li, Hung-Chih Kan, Jiunn-Yuan Lin, and Lai-Kwan Chau "Nanophotonic-enabled upconverting nanoparticle immunoassay for detecting ultralow concentrations of biomarkers in blood", Proc. SPIE PC13116, Nanoengineering: Fabrication, Properties, Optics, Thin Films, and Devices XXI, PC1311601 (4 October 2024); https://doi.org/10.1117/12.3026956

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KEYWORDS

Nanoparticles

Blood

Fluorescence

Molecules

Upconversion

Waveguides

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