Total internal reflection–based module for fluorescence and absorbance detection

Tom Verschooten; Heidi Ottevaere; Michael Vervaeke; Jürgen Van Erps; Hugo Thienpont

doi:10.1117/1.JMM.13.3.033001

3 July 2014 Total internal reflection–based module for fluorescence and absorbance detection

Tom Verschooten, Heidi Ottevaere, Michael Vervaeke, Jürgen Van Erps, Hugo Thienpont

Journal of Micro/Nanolithography, MEMS, and MOEMS, Vol. 13, Issue 3, 033001 (July 2014). https://doi.org/10.1117/1.JMM.13.3.033001

Abstract

We present a miniaturized polymer-based micro-optical detection unit for ultraviolet and visible laser-induced fluorescence (LIF) and absorbance (ABS) analysis with an interaction length of 3 mm. We use nonsequential optical ray tracing simulations to model the system and to optimize its performance with respect to optical efficiency and system complexity. The design features a compact optical system combining total internal reflection (TIR) mirrors and refractive optics. The detection module is prototyped with deep proton writing in 2-mm-thick polymethylmethacrylate and quantitatively characterized using a three-dimensional coordinate measurement machine. We demonstrate the proof-of-concept of this TIR mirror–based module for coumarin 480 obtaining limits of detection of 50 pM and 500 nM for LIF and ABS, respectively.

Citation Download Citation

Tom Verschooten, Heidi Ottevaere, Michael Vervaeke, Jürgen Van Erps, and Hugo Thienpont "Total internal reflection–based module for fluorescence and absorbance detection," Journal of Micro/Nanolithography, MEMS, and MOEMS 13(3), 033001 (3 July 2014). https://doi.org/10.1117/1.JMM.13.3.033001

Published: 3 July 2014

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