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1 April 2009 Frequency domain fluorimetry using a mercury vapor lamp
Matthew J. Bohn, Michael A. Lundin, Michael A. Marciniak
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Journal of Applied Remote Sensing, Vol. 3, Issue 1, 033524 (April 2009). https://doi.org/10.1117/1.3117448
Abstract
Frequency Domain (FD) fluorimetry, capitalizes on the frequency response function of a fluorophore and offers independence from light scatter and excitation/emission intensity variations in order to extract the sample's fluorescent lifetime. Mercury vapor lamps, a common source of industrial facility lighting, emit radiation that overlaps the UV/blue absorption spectrum of many fluorophores and may be used as an efficient and portable excitation source. The AC power modulation of mercury vapor lamps modulates the lamp's intensity at 120 Hz (in the United States) and higher harmonics. The fluorescent lifetimes for 3 different materials (willemite, uranium doped glass and U3O8) are measured with conventional techniques and compared with the FD technique using the power harmonics from a mercury vapor lamp. The mercury lamp measurements agree to within 25% of the conventional methods.
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Matthew J. Bohn, Michael A. Lundin, and Michael A. Marciniak "Frequency domain fluorimetry using a mercury vapor lamp," Journal of Applied Remote Sensing 3(1), 033524 (1 April 2009). https://doi.org/10.1117/1.3117448
Published: 1 April 2009
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Cited by 3 scholarly publications.
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KEYWORDS
Lamps
Mercury
Modulation
Sensors
Luminescence
Glasses
Remote sensing
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