In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography

Andrea Bassi; Luca Fieramonti; Cosimo D'Andrea; Gianluca Valentini; Marina Mione

doi:10.1117/1.3640808

1 October 2011 In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography

Andrea Bassi, Luca Fieramonti, Cosimo D'Andrea, Gianluca Valentini, Marina Mione

Author Affiliations +

Journal of Biomedical Optics, Vol. 16, Issue 10, 100502 (October 2011). https://doi.org/10.1117/1.3640808

Abstract

We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo, 3-D optical imaging technique that, without the use of any labeling, is able to reconstruct and visualize the vascular network of transparent and weakly scattering living specimens.

Citation Download Citation

Andrea Bassi, Luca Fieramonti, Cosimo D'Andrea, Gianluca Valentini, and Marina Mione "In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography," Journal of Biomedical Optics 16(10), 100502 (1 October 2011). https://doi.org/10.1117/1.3640808

Published: 1 October 2011

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY