Multifocus confocal Raman microspectroscopy for rapid single-particle analysis

Lingbo Kong; Pengfei Zhang; Yong-qing Li; Peter Setlow

doi:10.1117/1.3662456

1 December 2011 Multifocus confocal Raman microspectroscopy for rapid single-particle analysis

Lingbo Kong, Pengfei Zhang, Yong-qing Li, Peter Setlow

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Journal of Biomedical Optics, Vol. 16, Issue 12, 120503 (December 2011). https://doi.org/10.1117/1.3662456

Abstract

We have developed a multifocus confocal Raman microspectroscopy system that allows simultaneous analyses of ∼80 individual biological or airborne microparticles based on a precise image-guided technique. Multiple individual particles adhered in random positions on a coverslip were illuminated by a multifocus excitation pattern formed by rapidly steering a single laser beam with a pair of galvo-mirrors, and their Raman scatterings were synchronously projected with another galvo-mirror to different rows of a CCD chip for parallel spectroscopic analyses. We show that this technique can be used to rapidly identify single airborne particles or bacteria collected on a slide and to monitor germination dynamics of multiple bacterial spores in real-time.

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Lingbo Kong, Pengfei Zhang, Yong-qing Li, and Peter Setlow "Multifocus confocal Raman microspectroscopy for rapid single-particle analysis," Journal of Biomedical Optics 16(12), 120503 (1 December 2011). https://doi.org/10.1117/1.3662456

Published: 1 December 2011

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