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1 February 2011 Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy
Aaron D. Slepkov, Andrew Ridsdale, Adrian F. Pegoraro, Douglas J. Moffatt, John P. Pezacki, Albert Stolow, Huei-Ning Wan, Ming-Hao Wang, Fu-Jen Kao
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Journal of Biomedical Optics, Vol. 16, Issue 2, 021103 (February 2011). https://doi.org/10.1117/1.3490641
Abstract
We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.
©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Aaron D. Slepkov, Andrew Ridsdale, Adrian F. Pegoraro, Douglas J. Moffatt, John P. Pezacki, Albert Stolow, Huei-Ning Wan, Ming-Hao Wang, and Fu-Jen Kao "Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy," Journal of Biomedical Optics 16(2), 021103 (1 February 2011). https://doi.org/10.1117/1.3490641
Published: 1 February 2011
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KEYWORDS
Fluorescence lifetime imaging
Microscopes
Multimode fibers
Microscopy
Picosecond phenomena
CARS tomography
Sensors
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