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1.IntroductionThe transport of light in strongly scattering turbid media, such as biological tissue, is generally modeled as a diffusion process, which is described by the diffusion equation for the fluence .1,2 In condensed scattering media, is dependent on the refractive index , such that is conserved, enabling modeling of the diffusion of light in media with a spatially varying refractive index.3 However, a finite discontinuity in the refractive index, such as that which occurs at the boundary between two scattering media of differing refractive indices, gives rise to Fresnel reflection, such that is discontinuous, with a discontinuity proportional to the diffuse radiant flux at the boundary:4–7 where is a smoothly varying function of the index ratio tabulated as described previously.4The discontinuity in depends on the ratio of refractive indices at the boundary and is small for modest index ratios. Monte Carlo simulation of diffuse light transport across boundaries between turbid media with different refractive indices has shown that the error introduced when this correction is ignored is generally less than 10% for weakly absorbing scattering media, e.g., biological tissue illuminated with infrared radiation.6 An analytical solution for time-dependent diffusion between adjacent half-spaces presented previously8 supports this conclusion. However, the error is cumulative in multilayer scattering media and increases significantly for strongly forward-biased scattering and non-negligible absorption. A simple analytic method of assessing the error incurred in these circumstances when the discontinuity in is not taken into account would therefore be useful and is discussed below. Errors in modeling the diffuse fluence in turbid media can lead to systematic errors in diffuse transmittance and reflectance. They also introduce errors in scattering and absorption coefficients inferred from reflectance measurements and thus to errors in quantitative image reconstruction via diffuse optical tomography. Errors in estimating internal diffuse fluence may also impact on photodynamic therapy. 2.Theory2.1.Interface ErrorUsing the definitions previously published,9 where is the angular intensity distribution and is the direction cosine with respect to the positive -axis, we find the mean cosine of the radiant intensity distribution . Upon dividing Eq. (1) by , we find the relative error Similarly, Thus the error in applying the approximate boundary condition is directly proportional to the mean cosine of the angular intensity distribution at the boundary. This is illustrated in Fig. 1, where the ratio versus refractive index ratio for a given relative error (left-hand axis) and the relative error versus for a given ratio (right-hand axis) are plotted. Thus the larger the index ratio , the smaller the ratio required for a given relative error and the larger the relative error for a given ratio i.e., for a given angular radiance distribution with mean cosine .The discontinuity in diffuse fluence quantified by Eq. (1) also implies a discontinuity in the mean cosine of the radiance distribution at the boundary, viz., . To find the magnitude of the error in for a specific case requires numerical evaluation of the boundary fluxes.6 However, an estimate (lower-bound) can be made in terms of the mean cosine of the asymptotic angular radiance given by the following equation published previously:10 for scattering albedo , where is the scattering coefficient, is the absorption coefficient, is the extinction coefficient, and is the asymptotic attenuation coefficient. In the approximation,2 is replaced by , the effective attenuation coefficient. and the mean cosine are determined by the reduced scattering coefficient and the absorption coefficient , as follows: where , is the scattering asymmetry, the transport coefficient and is the diffusion coefficient.1 Thus , (isotropic radiance) when (zero absorption). More precise evaluation of the effective attenuation coefficient (and of , which is required for forward-biased scattering in absorbing media, involves higher moments of the phase function.11 In the approximation, the effective attenuation coefficient is as follows: where , , , , 2, 312,13 and the mean cosine of the asymptotic radiance The dependence of the relative error on scattering asymmetry is shown in Fig. 2 for scattering albedoes in the range for accurate values of .11 It can be seen that is only weakly dependent on scattering asymmetry for (backward-biased scattering), even for strong absorption (, i.e., ), while increasing rapidly for forward-biased scattering (), approaching 10% for when . In the approximation, the scattering asymmetry is reduced: for , but so is the scattering albedo: via the reduced scattering coefficient , potentially offsetting a reduced error in . The error in diffuse fluence increases for interfaces with higher index ratios, exceeding 20% for when .2.2.Diffusion EquationThe diffuse fluence obeys the steady-state diffusion equation1 where is the source distribution, is the diffusion coefficient, and is the effective attenuation coefficient. The radiant flux (net energy flow) is given by Fick’s law:1 Solutions of the diffusion equation, Eq. (7), subject to the boundary conditions, define the distribution of the diffuse fluence and radiant flux in scattering media with discontinuous refractive index.2.3.Boundary ConditionsThe boundary conditions at an interface between two diffusive scattering media with differing refractive indices , are as follows: for diffuse fluence , where and for (Ref. 4) and for conservation of radiant flux across the interface. These are applied to specific cases in Sec. 3 below. The error in diffuse fluence resulting from application of the approximate boundary condition14 is proportional to [Eq. (1)].2.4.Diffuse Fluence EquationsTo proceed further, solutions of the diffusion equation for two adjoining layers satisfying the boundary conditions [Eqs. (9a) and (9b)] are required. To simplify the analysis, consider planar asymptotic solutions for and in the respective scattering media:15 with effective attenuation coefficients , ; the -axis is taken perpendicular to the interface at . Upon inserting these solutions into Eqs. (9a) and (9b), we find where and assuming a semi-infinite medium (half-space) for , i.e., for as .Equations (12a), (12b), (12c), (12c′), (12d) enable comparison of the diffuse boundary fluences , with those satisfying the approximate boundary condition Eq. (10), which follow on setting in Eq. (12c), i.e., for . Analytic evaluation of the fractional flux error in terms of the refractive index ratio and the diffusion parameters , via the scattering asymmetry and scattering albedo can then be made. Accurate values of and for forward-biased anisotropic scattering in absorbing media () may be calculated from the phase function and scattering albedo .10 Alternatively, the mean cosine of the asymptotic radiance can be obtained from Eq. (4) and used in place of . Only need be calculated in this case, either analytically in the or approximations12,13 or numerically for higher accuracy.11 3.Results3.1.InterfaceThe boundary condition Eq. (9a) has been evaluated analytically by Shendeleva for time-dependent diffusion in adjoining media with isotropic scattering, and it has been validated by Monte Carlo simulation.3 Validation of the analytic method presented herein is provided by comparison with the results of Ripoll and Nieto-Vesperinas, who evaluated the error using numerical methods.6 Figure 3 shows the relative errors in the diffuse fluences at the common boundary between two adjoining media versus the refractive index of a scattering medium adjoining an aqueous scattering medium (), as calculated from the analytic formulae given above [Eqs. (12a), (12b), (12c), (12c′), (12d)]. The results show precise agreement with the numerical data (plotted points) published previously,6 confirming the validity of the simpler analytic method, which can therefore replace the previous numerical methods for rapid evaluation of the error in similar cases. Figure 4 shows the dependence of the fractional errors in the diffuse fluences versus scattering albedo for two adjoining media with disparate scattering parameters (, 0.95), calculated in the and approximations to the diffusion parameters for Henyey-Greenstein scattering,16 with refractive index ratio (tissue/aq). Initially, the error increases rapidly with absorption () for , reaching a broad maximum when ; in contrast, the error for in the adjoining medium increases quasilinearly to when . The results show that the approximation seriously underestimates the error in diffuse fluence (by ), whereas is low compared with the accurate value and is preferred for analytic evaluation of . Overall, the error increases sharply when there is non-negligible absorption in a turbid medium with strongly forward-biased scattering. 3.2.MultilayersFor diffusion of light in multiple layers of finite thickness, the diffuse fluence in the th layer may be expressed as previously described:15 with a similar expression for in the ()th layer. The boundary conditions described previously,4,7 yield the simple recurrence relations (for ), when , and , where , are the widths of the th and ()th layers, enabling the coefficients , to be related , . The results for the approximate boundary condition [Eq. (10)] are obtained upon setting in Eqs. (15a), (15b). Successive application of these relations yields the coefficients , for all the layers involved, with appropriate boundary conditions chosen for the first and last.15 A parallel set of coefficients, , , for , enables direct comparison of the accurate and approximate fluxes , in each layer and thus evaluation of the cumulative error for the multilayer system. This is illustrated in Fig. 5, with at a single interface (for , , ) and the cumulative error increasing with the total number of layers, exceeding 30% for five layers when . For multilayer media with higher index ratios, or for a larger number of layers, the cumulative error can easily exceed 100%.3.3.Perturbing LayerThe dependence of the diffuse reflectance of a layered medium on changes in the optical properties of a subsurface layer is of special interest.17 To illustrate this, consider a simple three-layer model comprising two-plane parallel layers supported on a semi-infinite layer (half-space) and vary the properties of the middle layer. The arrangement is sketched in Fig. 6. The optical properties are given in Table 1. The problem is analyzed via the equations for the diffuse fluences in the three regions: with the diffuse fluence decaying exponentially in the half-space, the flux in the midlayer having both exponential terms, and the flux in the surface layer including the exponential source for plane parallel illumination at the surface.2 The boundary conditions defined in Eqs. (14a) and (14b) are applied successively at the interfaces to evaluate the coefficients , for , 2, 3. The flux in the surface layer is extrapolated to zero a finite distance beyond the surface equal to the linear extrapolation distance determined by the refractive index .4 The radiant flux at the surface . A set of coefficients , for at each interface yields the uncorrected surface flux , allowing the relative error (and hence the relative error in diffuse reflectance) to be determined. A representative set of curves showing the dependence of on the ratio for selected values of the refractive index ratio is plotted in Fig. 7, with the relative error in surface flux increasing with and , reaching 30% for and , i.e., for (, ). In general, the error is larger (or smaller) for higher (or lower) values of scattering asymmetry and also increases with absorption.Table 1Optical properties of the three-layer system.
4.DiscussionThe analytic method of asymptotic planar fluxes enables straightforward evaluation of the error in diffuse fluence without recourse to a Monte Carlo simulation. The magnitude of the error is readily found from the optical properties of the adjoining scattering media, namely, the refractive index ratio, the reduced scattering coefficients, the absorption coefficients, and scattering asymmetries. It is simply expressed via the product , where is a monotonically increasing function of the index ratio (Ref. 4) and is the mean cosine of the boundary radiance, a key result of the above analysis. This is approximated by the asymptotic mean cosine of the radiance far from the boundary, expressed in terms of scattering albedo and diffuse attenuation coefficient . An equivalent formula for the error is , where is the diffusion coefficient. The dependence of flux error on scattering asymmetry is of some interest, viz., for forward-biased scattering in turbid media with near-negligible absorption (typical of biological tissue in the near-IR6). The error increases rapidly as , but is virtually independent of scattering asymmetry for negative values (Fig. 2). For forward-biased scattering media with non-negligible absorption, e.g., biological media in the visible spectrum, the error becomes progressively less dependent on scattering asymmetry as absorption increases, ultimately becoming independent of in the limit of zero scattering albedo . Having found a simple means of estimating the magnitude of the flux error at a boundary, accurate formulae for the diffuse fluence in adjoining media incorporating the correction term are obtained from the boundary condition Eq. (1). It is noted that the correction applies to the diffuse fluence distribution throughout the turbid medium, not simply at the boundaries. In the case of multilayer media, repeated application of the boundary conditions yields recurrence relations for the coefficients , of the formula for the diffuse fluence. A corresponding set of coefficients , obtained from the approximate boundary condition [with ] enables (1) the flux error in successive layers to be obtained and (2) its parametric dependence on the optical properties of the layers to be investigated. This is of importance for comparison with experimental determination of diffuse reflectance, as well as for the inverse problem of determining optical constants from reflectance measurements. The principal aim of the present work is to provide a simple analytic method for estimating the error incurred in using the approximate form of the boundary condition [Eq. (10)]. This has been checked against the computational results reported previously6 (Sec. 3.1 and Fig. 3) and illustrated with several examples relevant to biomedical optics. The work presented herein concerns analysis of the error involved in applying the approximate diffusion boundary condition [Eq. (10)] rather than the error in using diffusion theory per se. Thus a numerical evaluation would merely quantify the “error within the error,” whereas the analytic method provides a simple means of estimating its magnitude. The analytic approach was never intended to replace accurate radiative transfer computations where these are merited, e.g., Phillips and Jacques,17 but rather as a simple check on the diffusion approximation, e.g., the widely used formulation.2 5.ConclusionA simple analytic method of estimating the error involved in applying a commonly used approximate boundary condition for diffuse radiation in two adjoining scattering media with differing refractive indices has been presented. The method is based on asymptotic planar fluxes and enables the relative error to be readily evaluated without recourse to Monte Carlo simulation. Three examples of its application have been considered: (1) evaluating the error in calculating the diffuse fluences at a boundary between two media with differing refractive indices and dissimilar scattering properties, (2) the dependence of the relative error in diffuse fluence in a multilayer medium with discontinuous refractive index on the ratio of the reduced scattering coefficient to the absorption coefficient , and (3) the dependence of the relative error in radiant flux at the surface of a three-layer medium on the ratio in the middle layer. In addition to its dependence on refractive index ratio via the function , the fluence error increases with scattering asymmetry in forward-biased scattering media and is cumulative in multilayered media with refractive index increments between layers. The same methodology can be applied to multilayer problems with cylindrical symmetry, with the system being first converted to planar geometry via a Hankel transform, to allow 1-D analysis (as here), followed by reconversion of the solution to cylindrical symmetry via an inverse Hankel transform.15 ReferencesR. C. Haskellet al.,
“Boundary conditions for the diffusion equation in radiative transfer,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 11
(10), 2727
–2741
(1994). http://dx.doi.org/10.1364/JOSAA.11.002727 JOAOD6 1084-7529 Google Scholar
S. A. CarpS. A. PrahlV. Venugopalan,
“Radiative transport in the approximation: accuracy of fluence rate and optical penetration depth predictions in turbid semi-infinite media,”
J. Biomed. Opt., 9
(3), 632
–647
(2004). http://dx.doi.org/10.1117/1.1695412 JBOPFO 1083-3668 Google Scholar
M. L. Shendeleva,
“Radiative transfer in a turbid medium with a varying refractive index: comment,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 21
(12), 2464
–2467
(2004). http://dx.doi.org/10.1364/JOSAA.21.002464 JOAOD6 1084-7529 Google Scholar
R. Aronson,
“Boundary conditions for diffusion of light,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 12
(11), 2532
–2539
(1995). http://dx.doi.org/10.1364/JOSAA.12.002532 JOAOD6 1084-7529 Google Scholar
G. W. Faris,
“Diffusion equation boundary conditions for the interface between turbid media: a comment,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 19
(3), 519
–520
(2002). http://dx.doi.org/10.1364/JOSAA.19.000519 JOAOD6 1084-7529 Google Scholar
J. RipollM. Nieto-Vesperinas,
“Index mismatch for diffuse photon density waves at both flat and rough diffuse—diffuse interfaces,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 16
(8), 1947
–1957
(1999). http://dx.doi.org/10.1364/JOSAA.16.001947 JOAOD6 1084-7529 Google Scholar
J. S. CassellM. M. R. Williams,
“Radiation transport and internal reflection in a two region, turbid sphere,”
J. Quant. Spectrosc. Radiat. Transf., 104
(3), 400
–427
(2007). http://dx.doi.org/10.1016/j.jqsrt.2006.09.007 JQSRAE 0022-4073 Google Scholar
M. L. Shendeleva,
“Influence of boundary conditions on photon diffusion through an interface between two turbid media with different refractive indices,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 27
(7), 1521
–1528
(2010). http://dx.doi.org/10.1364/JOSAA.27.001521 JOAOD6 1084-7529 Google Scholar
S. Chandrasekhar, Radiative Transfer, Dover, New York
(1960). Google Scholar
V. I. Haltrin,
“Exact solution of the characteristic equation for transfer in the anisotropically scattering and absorbing medium,”
Appl. Opt., 27
(3), 599
–602
(1988). http://dx.doi.org/10.1364/AO.27.000599 APOPAI 0003-6935 Google Scholar
D. C. SahniE. B. DahlN. G. Sjöstrand,
“Diffusion coefficient for photon transport in turbid media,”
Phys. Med. Biol., 48
(23), 3969
–3976
(2003). http://dx.doi.org/10.1088/0031-9155/48/23/012 PHMBA7 0031-9155 Google Scholar
W. M. Star,
“Comparing the P3-approximation with diffusion theory and with Monte Carlo calculations of light propagation in a slab geometry,”
Dosimetry of Laser Radiation in Medicine and Biology, IS5 146
–154 SPIE Optical Engineering Press, Bellingham, WA
(1989). Google Scholar
E. L. HullT. H. Foster,
“Steady-state reflectance spectroscopy in the approximation,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 18
(3), 584
–599
(2001). http://dx.doi.org/10.1364/JOSAA.18.000584 JOAOD6 1084-7529 Google Scholar
J.-M. Tualleet al.,
“Real-space Green’s function calculation for the solution of the diffusion equation in stratified turbid media,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 17
(11), 2046
–2055
(2000). http://dx.doi.org/10.1364/JOSAA.17.002046 JOAOD6 1084-7529 Google Scholar
A. LiemertA. Kienle,
“Light diffusion in a turbid cylinder. II. Layered case,”
Opt. Express, 18
(9), 9266
–9279
(2010). OPEXFF 1094-4087 Google Scholar
L. G. HenyeyJ. A. Greenstein,
“Diffuse radiation in the galaxy,”
Astrophys. J., 93 70
–83
(1941). http://dx.doi.org/10.1086/144246 ASJOAB 0004-637X Google Scholar
K. G. PhillipsS. L. Jacques,
“Solution of transport equations in layered media with refractive index mismatch using the PN-method,”
J. Opt. Soc. Am. A Opt. Image Sci. Vis., 26
(10), 2147
–2162
(2009). http://dx.doi.org/10.1364/JOSAA.26.002147 JOAOD6 1084-7529 Google Scholar
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