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22 March 2013 Purple sea urchin Strongylocentrotus purpuratus gamete manipulation using optical trapping and microfluidics
Charlie Chandsawangbhuwana, Linda Z. Shi, Qingyuan Zhu, Michael W. Berns
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Journal of Biomedical Optics, Vol. 18, Issue 4, 040501 (March 2013). https://doi.org/10.1117/1.JBO.18.4.040501
Abstract
A system has been developed that allows for optical and fluidic manipulation of gametes. The optical manipulation is performed by using a single-point gradient trap with a 40× oil immersion PH3 1.3 NA objective on a Zeiss inverted microscope. The fluidic manipulation is performed by using a custom microfluidic chamber designed to fit into the short working distance between the condenser and objective. The system is validated using purple sea urchin Strongylocentrotus purpuratus gametes and has the potential to be used for mammalian in vitro fertilization and animal husbandry.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Charlie Chandsawangbhuwana, Linda Z. Shi, Qingyuan Zhu, and Michael W. Berns "Purple sea urchin Strongylocentrotus purpuratus gamete manipulation using optical trapping and microfluidics," Journal of Biomedical Optics 18(4), 040501 (22 March 2013). https://doi.org/10.1117/1.JBO.18.4.040501
Published: 22 March 2013
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KEYWORDS
Microfluidics
Optical tweezers
In vitro testing
Lanthanum
Microscopes
Silicon
Ocean optics
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