Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer

Joey M. Jabbour; Shuna Cheng; Bilal H. Malik; Rodrigo Cuenca; Javier A. Jo; John Wright D.D.S.; Yi-Shing L. Cheng D.D.S.; Kristen C. Maitland

doi:10.1117/1.JBO.18.4.046012

17 April 2013 Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer

Joey M. Jabbour, Shuna Cheng, Bilal H. Malik, Rodrigo Cuenca, Javier A. Jo, John Wright D.D.S., Yi-Shing L. Cheng D.D.S., Kristen C. Maitland

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Journal of Biomedical Optics, Vol. 18, Issue 4, 046012 (April 2013). https://doi.org/10.1117/1.JBO.18.4.046012

Abstract

Optical imaging techniques using a variety of contrast mechanisms are under evaluation for early detection of epithelial precancer; however, tradeoffs in field of view (FOV) and resolution may limit their application. Therefore, we present a multiscale multimodal optical imaging system combining macroscopic biochemical imaging of fluorescence lifetime imaging (FLIM) with subcellular morphologic imaging of reflectance confocal microscopy (RCM). The FLIM module images a 16×16 mm ² tissue area with 62.5 μm lateral and 320 ps temporal resolution to guide cellular imaging of suspicious regions. Subsequently, coregistered RCM images are acquired at 7 Hz with 400 μm diameter FOV, <1 μm lateral and 3.5 μm axial resolution. FLIM-RCM imaging was performed on a tissue phantom, normal porcine buccal mucosa, and a hamster cheek pouch model of oral carcinogenesis. While FLIM is sensitive to biochemical and macroscopic architectural changes in tissue, RCM provides images of cell nuclear morphology, all key indicators of precancer progression.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Joey M. Jabbour, Shuna Cheng, Bilal H. Malik, Rodrigo Cuenca, Javier A. Jo, John Wright D.D.S., Yi-Shing L. Cheng D.D.S., and Kristen C. Maitland "Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer," Journal of Biomedical Optics 18(4), 046012 (17 April 2013). https://doi.org/10.1117/1.JBO.18.4.046012

Published: 17 April 2013

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