Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis

Jing-gao Zheng; Tiancheng Huo; Ning Tian; Tianyuan Chen; Chengming Wang; Ning Zhang; Fengying Zhao; Danyu Lu; Dieyan Chen; Wanyun Ma; Jia-Lin Sun; Ping Xue

doi:10.1117/1.JBO.18.5.050505

9 May 2013 Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis

Jing-gao Zheng, Tiancheng Huo, Ning Tian, Tianyuan Chen, Chengming Wang, Ning Zhang, Fengying Zhao, Danyu Lu, Dieyan Chen, Wanyun Ma, Jia-Lin Sun, Ping Xue

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Journal of Biomedical Optics, Vol. 18, Issue 5, 050505 (May 2013). https://doi.org/10.1117/1.JBO.18.5.050505

Abstract

The spindle plays a crucial role in normal chromosome alignment and segregation during meiosis and mitosis. Studying spindles in living cells noninvasively is of great value in assisted reproduction technology (ART). Here, we present a novel spindle imaging methodology, full-field optical coherence tomography (FF-OCT). Without any dye labeling and fixation, we demonstrate the first successful application of FF-OCT to noninvasive three-dimensional (3-D) live imaging of the meiotic spindles within the mouse living oocytes at metaphase II as well as the mitotic spindles in the living zygotes at metaphase and telophase. By post-processing of the 3-D dataset obtained with FF-OCT, the important morphological and spatial parameters of the spindles, such as short and long axes, spatial localization, and the angle of meiotic spindle deviation from the first polar body in the oocyte were precisely measured with the spatial resolution of 0.7 μm. Our results reveal the potential of FF-OCT as an imaging tool capable of noninvasive 3-D live morphological analysis for spindles, which might be useful to ART related procedures and many other spindle related studies.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Jing-gao Zheng, Tiancheng Huo, Ning Tian, Tianyuan Chen, Chengming Wang, Ning Zhang, Fengying Zhao, Danyu Lu, Dieyan Chen, Wanyun Ma, Jia-Lin Sun, and Ping Xue "Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis," Journal of Biomedical Optics 18(5), 050505 (9 May 2013). https://doi.org/10.1117/1.JBO.18.5.050505

Published: 9 May 2013

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