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15 September 2014 Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing
Tae-Youl Choi, Michael L. Denton, Gary D. Noojin, Larry E. Estlack, Ramesh L. Shrestha, Benjamin A. Rockwell, Robert J. Thomas, Dongsik Kim
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Journal of Biomedical Optics, Vol. 19, Issue 9, 097003 (September 2014). https://doi.org/10.1117/1.JBO.19.9.097003
Abstract
A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-μm beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipette-based thermal sensors of size ranging from 2 to 4  μm into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73°C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2014/$25.00 © 2014 SPIE
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Tae-Youl Choi, Michael L. Denton, Gary D. Noojin, Larry E. Estlack, Ramesh L. Shrestha, Benjamin A. Rockwell, Robert J. Thomas, and Dongsik Kim "Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing," Journal of Biomedical Optics 19(9), 097003 (15 September 2014). https://doi.org/10.1117/1.JBO.19.9.097003
Published: 15 September 2014
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Cited by 2 scholarly publications and 1 patent.
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KEYWORDS
Temperature metrology
Sensors
Luminescence
In vitro testing
Thermal modeling
Thermal sensing
Infrared sensors
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