Self-guided reconstruction for time-domain fluorescence molecular lifetime tomography

Chuangjian Cai; Wenjuan Cai; Jiaju Cheng; Yuxuan Yang; Jianwen Luo

doi:10.1117/1.JBO.21.12.126012

21 December 2016 Self-guided reconstruction for time-domain fluorescence molecular lifetime tomography

Chuangjian Cai, Wenjuan Cai, Jiaju Cheng, Yuxuan Yang, Jianwen Luo

Author Affiliations +

Journal of Biomedical Optics, Vol. 21, Issue 12, 126012 (December 2016). https://doi.org/10.1117/1.JBO.21.12.126012

Abstract

Fluorescence probes have distinct yields and lifetimes when located in different environments, which makes the reconstruction of fluorescence molecular lifetime tomography (FMLT) challenging. To enhance the reconstruction performance of time-domain (TD) FMLT with heterogeneous targets, a self-guided L1 regularization projected steepest descent (SGL1PSD) algorithm is proposed. Different from other algorithms performed in time domain, SGL1PSD introduces a time-resolved strategy into fluorescence yield reconstruction. The algorithm consists of four steps. Step 1 reconstructs the initial yield map with full time gate strategy; steps 2–4 reconstruct the inverse lifetime map, the yield map, and the inverse lifetime map again with time-resolved strategy, respectively. The reconstruction result of each step is used as a priori for the reconstruction of the next step. Projected iterated Tikhonov regularization algorithm is adopted for the yield map reconstructions in steps 1 and 3 to provide a solution with iterative refinement and nonnegative constraint. The inverse lifetime map reconstructions in steps 2 and 4 are based on L1 regularization projected steepest descent algorithm, which employ the L1 regularization to reduce the ill-posedness of the high-dimensional nonlinear problem. Phantom experiments with heterogeneous targets at different edge-to-edge distances demonstrate that SGL1PSD can provide high resolution and quantification accuracy for TD FMLT.

Citation Download Citation

Chuangjian Cai, Wenjuan Cai, Jiaju Cheng, Yuxuan Yang, and Jianwen Luo "Self-guided reconstruction for time-domain fluorescence molecular lifetime tomography," Journal of Biomedical Optics 21(12), 126012 (21 December 2016). https://doi.org/10.1117/1.JBO.21.12.126012

Received: 11 September 2016; Accepted: 30 November 2016; Published: 21 December 2016

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 4 scholarly publications.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Reconstruction algorithms

Atrial fibrillation

Luminescence

Tomography

Fluorescence tomography

Detection and tracking algorithms

Sensors

Show All Keywords

RELATED CONTENT

Depth compensation in fluorescence molecular tomography using an adaptive support...
Proceedings of SPIE (September 19 2014)

Cherenkov-excited luminescence sheet imaging (CELSI) tomographic reconstruction
Proceedings of SPIE (February 08 2017)

The improved reconstruction of fluorescence molecular tomography via regularized doubly...
Proceedings of SPIE (March 10 2020)

Analysis of reconstructions in full view fluorescence molecular tomography
Proceedings of SPIE (February 28 2007)

Fluorescence molecular tomography reconstruction using hybrid regularization method
Proceedings of SPIE (August 22 2011)

Toward robust high resolution fluorescence tomography a hybrid row...
Proceedings of SPIE (March 01 2011)

A CT-analogous method for high-resolution fluorescence molecular tomography
Proceedings of SPIE (February 29 2012)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years