Longitudinal in vivo imaging of perineuronal nets

Amit Benbenishty; Shany Peled-Hajaj; Venkat Raghavan Krishnaswamy; Hagai Har-Gil; Sapir Havusha-Laufer; Antonella Ruggiero; Inna Slutsky; Pablo Blinder; Irit Sagi

doi:10.1117/1.NPh.10.1.015008

24 March 2023 Longitudinal in vivo imaging of perineuronal nets

Amit Benbenishty, Shany Peled-Hajaj, Venkat Raghavan Krishnaswamy, Hagai Har-Gil, Sapir Havusha-Laufer, Antonella Ruggiero, Inna Slutsky, Pablo Blinder, Irit Sagi

Author Affiliations +

Neurophotonics, Vol. 10, Issue 1, 015008 (March 2023). https://doi.org/10.1117/1.NPh.10.1.015008

Abstract

Significance

Perineuronal nets (PNNs) are extracellular matrix structures implicated in learning, memory, information processing, synaptic plasticity, and neuroprotection. However, our understanding of mechanisms governing the evidently important contribution of PNNs to central nervous system function is lacking. A primary cause for this gap of knowledge is the absence of direct experimental tools to study their role in vivo.

Aim

We introduce a robust approach for quantitative longitudinal imaging of PNNs in brains of awake mice at subcellular resolution.

Approach

We label PNNs in vivo with commercially available compounds and monitor their dynamics with two-photon imaging.

Results

Using our approach, we show that it is possible to longitudinally follow the same PNNs in vivo while monitoring degradation and reconstitution of PNNs. We demonstrate the compatibility of our method to simultaneously monitor neuronal calcium dynamics in vivo and compare the activity of neurons with and without PNNs.

Conclusion

Our approach is tailored for studying the intricate role of PNNs in vivo, while paving the road for elucidating their role in different neuropathological conditions.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Amit Benbenishty, Shany Peled-Hajaj, Venkat Raghavan Krishnaswamy, Hagai Har-Gil, Sapir Havusha-Laufer, Antonella Ruggiero, Inna Slutsky, Pablo Blinder, and Irit Sagi "Longitudinal in vivo imaging of perineuronal nets," Neurophotonics 10(1), 015008 (24 March 2023). https://doi.org/10.1117/1.NPh.10.1.015008

Received: 8 October 2022; Accepted: 23 February 2023; Published: 24 March 2023

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
13 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY