The light source in digital lensless holographic microscopy (DLHM) plays a key role in the optical performance of this microscopy methodology. The underlying physics that support DLHM rely on having an outward propagating spherical wavefront illuminating the sample to record on the surface of a digital camera the intensity of the diffracted wavefield that is magnified by free-space propagation; this first stage constitutes the recording. The information of the sample is numerically retrieved by computing the diffraction that an inward propagating spherical wavefront undergoes in the recorded intensity in a stage known as reconstruction. In both stages of this lensless microscopy method, it is assumed that perfect spherical wavefronts are utilized; hence, the closer the illumination light sources to |
ACCESS THE FULL ARTICLE
No SPIE Account? Create one
Wavefronts
Light sources and illumination
Aspheric lenses
Optical fibers
Spherical lenses
Digital holography
Light sources